Interaction of Rice Quantitative Trait Locus gw9.1 with Three Grain Shape Genes

Abstract

Grain size is one of the most important factors determining grain yield in rice breeding. In previous studies, we constructed high-density maps for two quantitative trait loci (QTL) for grain weight, tgw2 and gw9.1, using progeny derived from crosses between the japonica cultivar Hwaseong and Oryza grandiglumis, and Hwaseong and O. rufipogon (IRGC 105491), respectively. The wild alleles contributed an increase in grain weight at these two loci. We developed an F2 population (146 plants) by crossing two near isogenic lines (NILs) harboring tgw2 and gw9.1 to know how they interact in the near isogenic background. Simple sequence repeat markers tightly linked to two QTL were used to check the genotype of the F2 population. Based on the genotype at two loci, 146 F2 plants were classified into 9 groups with a combination of three genotypes at each two loci. Two gene interaction was not significant (P=0.99) in the F2. Homozygous plants with wild alleles at two loci showed significantly higher 1,000 grain weight than plants with a single QTL in the F2 and F3. These results indicate that two QTLs act additively in distinct or complementary pathways in controlling GW. Gene expression analysis was also performed to know the relationship of the gw9.1 QTL with three major grain size genes with Hwaseong and two NILs plants at the transcription level. The results from this study provide insight into grain size regulation in rice and are likely to be useful for marker aided selection for grain size.