Abstract
Membranes isolated from vegetative cells of Anabaena variabilis (ATCC 29413) oxidine NADPH and NADH in a cyanide‐sensitive reaction, NADPH being the better donor. In addition, both act as reductants for photosystem I in the light and in the presence of DCMU. An NADH‐regenerating system has been introduced experimentally, functioning as donor system for photosystem I of Anabaena membranes. This NADH dehydrogenation is strictly light‐dependent, sensitive to DBMIB, establishes a transhydrogenase system with NADP+ was hydrogen acceptor and competes with oxygen. Kinetic analysis of oxygen uptake with reference to nucleotide concentration exhibited different Km‐ and Vmax‐values for NADPH and NADH in the light and in the dark.
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