Interaction of nucleoside diphosphate kinase and catalases for stress and light responses in Neurospora crassa

Abstract

Nucleoside diphosphate kinase (NDK) is an ubiquitous enzyme with the function of a signal transducer. In Neurospora crassa, an ndk-1 P72 H mutant carrying the point mutation Pro72His was isolated. We found that ndk-1 P72 H showed hypersensitivity to oxidative and heat stress and a decrease in the levels of catalase (Cat)-1 and -3 induced by oxidative, heat stress and illumination compared with wild type (Wt). We found, by conducting a yeast two-hybrid assay, that Cat-1 interacted with NDK-1. NDK-1 was suggested to control Cat-1 and Cat-3 at the post-transcriptional level in response to heat, oxidative stress and light.