Abstract
Binding isotherms of N-phenyl-1-naphthylamine to different lipid monolayers are obtained by use of fluorescence spectroscopy for different surface pressures. In the case of dipalmitoyl phosphatidylcholine, the affinity is high in the liquid expanded state, but drops in the liquid condensed state. The affinity for the negatively charged dilauryl phosphatidylglycerol is very low. In the case of phosphatidylethanolamine extracted from Escherichia coli, a strong decrease in the affinity is detected about 20 mN/m. This can be explained by an increase of interactions between the molecules of the lipid matrix. These data lead to the conclusion that the packing is not uniform in sonicated vesicles.Key words: monolayer, phospholipid, fluorescence, phase transition.
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