Abstract
The interaction between insulin molecule and several lipid monolayers was studied. Insulin interaction with lipid films gave rise to an increase in surface pressure. A linear relationship between the change in surface pressure, ΔII, and the lipid II was observed for all the lipids studied. The limiting II, i.e., when the lipid II is of such magnitude that protein can no longer penetrate (ΔII = 0), was found to be much larger in the case of cholesterol than for the other lipid films. The magnitude of limiting II in the various lipid films is explained through the action of van der Waals forces acting between the methylene groups of the hydrocarbon chains of neighbor molecules.
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