Abstract
Abstract Rat leukemic basophils with surface-bound IgE were reacted with fluoresceinated anti-IgE. The results were qualitatively similar to those obtained in analogous studies with lymphocytes, normal human basophils, and other cells: local aggregation (patch formation) was only moderately temperature-dependent and was uninhibited by NaN3. Polar cap formation was significantly more sensitive to temperature and was completely inhibited by 0.01 to 0.1 M NaN3 and cytochalasin B. The amount and rate of capping could be altered by varying either the number of bound IgE molecules or the anti-IgE concentration indicating that lattice formation is required for redistribution. Monovalent Fab′ fragments of the anti-IgE caused no redistribution. No endocytosis of the antibody-surface determinant complexes was observed. Under conditions adequate to inhibit cap formation on mouse lymphocytes, concanavalin A did not inhibit cap formation on the leukemic basophils.
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