Abstract
Cetylpyridinium chloride (CPC) is commonly used to precipitate hyaluronic acid (HA) from crude extract in a HA purification process. 3‐(Trimethoxysilyl)‐propyldimethyl octadecyl ammonium chloride (Si‐QAC) has a structure very similar to CPC when it is bonded to surfaces through its silane base. By taking advantage of its easy surface bonding property, Si‐QAC was bonded to the silica‐coated magnetite to facilitate HA recovery. The effects of pH, salt concentration, and the temperature on HA adsorption to Si‐QAC‐magnetite were studied. Not only the positively charged nitrogen but also hydrophobic long tail of Si‐QAC contributed to the strong interaction between HA and Si‐QAC‐magnetite. The maximum HA adsorption capacity was 38 mg/g‐dry magnetite and the equilibrium dissociation constant was 0.016 g/l at pH 4 under 37°C. The adsorbed HA could be effectively desorbed by 1 N NaCl supplemented with 0.1% Triton X100.
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