Interaction of glutathione S- transferase M1 and T1 gene polymorphism and oxidative stress associated biomarkers (GSH and GST) in pesticide exposed workers in Himachal Pradesh

Abstract

Humans get oxidative stress as a result of using several pesticides and herbicides in excess. Occupational and environmental exposures harm the public’s health even if their effectiveness has led to their widespread use. The current study’s goal was to assess the activity of the antioxidants glutathione reductase and glutathione S-transferase in a group of HP, India residents who had been exposed to pesticides. Pesticide exposed individuals had their glutathione transferase (GSTM1 and GSTT1) genetic polymorphisms investigated to check for any connections to oxidative stress and toxicity. For the genotyping of GSTM1, GSTT1, and antioxidant enzymatic assays such as GSH and GST, 5 ml of blood was drawn from the population under study. Antioxidant enzymatic activity of GSH (205.63 ± 42.42) and GST (1.65 ± 0.39) were decreased in pesticide exposed group as compared to unexposed group GSH (273.76 ± 56.70) and GST (2.40 ± 0.81). We observed non-significant (p>0.05) results for the association of GSTM1 with GSH and GST enzyme activity as well as similar result have been observed for the association of GSTT1 with GSH and GST activity in the studied population. According to the study, prolonged pesticide exposure results in oxidative stress, which is connected to the necessary genetic variance. The findings may pave the way for further investigation into the toxicogenetics and health impacts of pesticides.