Abstract
INTERACTION OF ESTROGEN AND THYROID HORMONES IN THE NUCLEI OF UTERINE TISSUE. Zorica Žakula, S. Đurica, S. Radivojsa, T. Milosavljevic and Nevena Ribarac-Stepic. Department of Molecular Biology and Endocrinology, Vinca Institute of Nuclear Sciences, 11001 Belgrade, Serbia and Montenegro; Internal Medicine Clinic Zvezdara, University Clinical Centre, Medical Faculty, University of Belgrade, 11000 Belgrade, Serbia and Montenegro
Highlights
Thyroid hormone and estradiol modulate biological processes by binding to nuclear receptor proteins that, through interactions with specific response elements in the regulatory regions of genes, modulate gene transcription
In order to gain more information about the relationship between thyroid and estrogen hormone actions, we studied receptor binding parameters for both kinds of analyzed hormones, as well as the competition in binding of these hormones to nuclear receptors in the rat uterus
The animals were killed by decapitation, and the uteri were minced and homogenized in TEMG buffer (50 mM TrisHCl, 1 mM EDTA, 12 mM monothioglycerol, 30% glycerol, pH 7.5) using an Ultra-Turrax homogenizer (3x30 sec)
Summary
Thyroid hormone and estradiol modulate biological processes by binding to nuclear receptor proteins that, through interactions with specific response elements in the regulatory regions of genes, modulate gene transcription. In order to gain more information about the relationship between thyroid and estrogen hormone actions, we studied receptor binding parameters for both kinds of analyzed hormones, as well as the competition in binding of these hormones to nuclear receptors in the rat uterus. After centrifugation at 100.000xg for 1 h at 40C, the obtained supernatant (nuclear extract) was used in the binding studies.
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