Interaction of divalent cations with germ cell specific sulfogalactosylglycerolipid and the effects on lipid chain dynamics.

Abstract

Sulfogalactosylglycerolipid (SGG) is a sulfoglycolipid found ubiquitously in the plasma membrane of mammalian male germ cells. Although its exact cellular function(s) is unknown, it is speculated that SGG may play a role in cation transport, which may be important in sperm-egg interaction. Given the significant role of Ca2+ in many fertilization-related events, the purpose of this study was to determine whether Ca2+ interaction with the negatively charged sulfate group of SGG results in changes to the SGG lipid chain molecular dynamics and to compare these lipid dynamics with those resulting from Na+, Mg2+, or Sr2+ interaction with SGG. Pressure-tuning Fourier transform infrared spectroscopy was used in this study. The results obtained showed that all three divalent cations interacted electrostatically with the sulfate moiety of hydrated SGG, although with varying degrees of strength. It was found that the hydrocarbon chains of hydrated SGG-Na+ multilamellar bilayers were interdigitated, thus increasing disorderedness of the terminal CH3 group of the hydrocarbon chains. The presence of each of the three divalent cations abolished this interdigitation state. Presumably, this is through the cross-linking interaction of each divalent cation with the sulfate groups of neighboring lipid molecules. Moreover, divalent cation interaction was found to increase the lipid chain dynamics of SGG, with Mg2+ inducing the greatest chain disorder followed by Ca2+ and then Sr2+. An increase in chain disorder would increase the bilayer fluidity. Such a phenomenon may prove relevant to the changes observed in the sperm plasma membrane during fertilization-related events.