Interaction of discoidal complexes of dimyristoyl phosphatidylcholine-cholesterol-apolipoprotein A-I with human plasma high density lipoprotein HDL3.

Abstract

The interaction of human plasma high density lipoproteins (HDL3) with discoidal complexes of apolipoprotein A-I (apo A-I) and dimyristoyl phosphatidylcholine (DMPC) containing 0, 10, 20 or 30 mol % cholesterol was investigated. Discoidal complexes containing various amounts of cholesterol were prepared by incubating apo A-I and DMPC-cholesterol liposomes for 12 hr at 25 C; the protein-lipid complexes were isolated by gel filtration chromatography on Bio-Gel A15m. Increasing the cholesterol content from 0 to 30 mol % caused a decrease in the fluidity of the discoidal complexes as determined by fluorescence polarization with 1,6-diphenyl-1,3,5-hexatriene; a reduced phase-transition amplitude; a decrease in the ratio of apo A-I to DMPC; and an increase in the width of the discoidal complexes as determined by electron microscopy after negative staining. Incubation of the apo A-I-lipid complexes with HDL3 resulted in a complete breakdown of the discoidal structures and a transfer to DMPC and cholesterol to HDL3. As a result of lipid transfer, there was an increase in the size of HDL3. These in vitro results may be of significance as they relate to the interconversion of HDL subfractions during lipoprotein-lipase-induced lipolysis of triglyceride-rich lipoproteins.