Interaction of deoxyhalosucrose derivatives with dextransucrase

Abstract

Members of a series of deoxyhalosucrose analogs substituted at one, two, or three primary carbon atoms with bromine or chlorine were prepared. Dextransucrase isolated from Streptococcus sanguis was separately treated with 6-bromo-6-deoxysucrose, 6,6′-dibromo-6,6′-dideoxysucrose, 6,1′,6′-tribromotrideoxysucrose, and 6,6′-dichlorodideoxysucrose, in order to determine if they were inactivators. Variation in time of exposure, and in the concentration of the sucrose analogs, did not yield significant irreversible inactivation. In supplementary studies, it was found that the compounds serve as weak, reversible inhibitors.