Abstract
Background and Aims: Our study aimed to evaluate how curcumin affect cisplatin cytotoxicity in human cervical carcinoma (HeLa), human hepatocellular carcinoma (HepG2), and Chinese hamster lung fibroblast (V79) cells. Methods: The cytotoxicity was evaluated by MTT assay. Results: The IC50 values of curcumin were 404 μM and 320 μM in HeLa cells; 236 μM and 98.3 μM in HepG2 cells; 877 μM and 119 μM in V79 cells; for 24 h and 48 h, respectively. The IC50 values of cisplatin were 22.4 μM and 12.3 μM in HeLa cells; 25.5 μM and 7.7 μM in HepG2 cells; 15.4 μM and 4.9 μM in V79 cells; for 24 h and 48 h, respectively. Curcumin significantly decreased cisplatin cytotoxicity at 500 μM in HeLa cells and above 250 μM and 125 μM in HepG2 cells, for 24 h and 48 h, respectively. In V79 cells, curcumin significantly decreased the IC50 values of cisplatin above 500 μM and 125 μM for 24 h and 48 h. Conclusion: The results might contribute to the anticancer effect of the curcumin-cisplatin combination in cervical and hepatocellular carcinoma, but in order to support this result and determine its interactions with antineoplastic drugs, further studies are needed.
Highlights
Multidisciplinary treatments, including surgical treatment, radiotherapy and chemotherapy are applied to patients with cancer
Chemicals The chemicals used in the experiments were purchased from the following suppliers: cisplatin (Koçak Farma, Turkey); curcumin, dimethyl sulfoxide (DMSO), Dublecco's modified Eagle's medium (DMEM), ethanol, fetal bovine serum (FBS), MTT, penicillin-streptomycin, trypan blue, trypsin–EDTA, RPMI 1640 medium, Dulbecco’s phosphate buffered saline (PBS) from Sigma
Cisplatin did not cause significant cytotoxic effect at the concentrations of 0.49-7.81 μM and at the concentrations of 0.49-3.91 μM when compared to the negative control for 24 h and 48 h, respectively; the cell viabilities were significantly decreased above 15.63 μM and 7.81 μM of cisplatin for 24 h and 48 h incubation, respectively, in a dose-dependent manner (p
Summary
Multidisciplinary treatments, including surgical treatment, radiotherapy and chemotherapy are applied to patients with cancer. One of the chemotherapeutic agents, is often used in the treatment of solid tumors such as testis, over, bladder, prostate, cervix, and lung cancer (Rosenberg, 1985). It has severely dose-limiting toxicity including ototoxicity, neurotoxicity, nephrotoxicity and cardiotoxicity. Curcumin significantly decreased cisplatin cytotoxicity at 500 μM in HeLa cells and above 250 μM and 125 μM in HepG2 cells, for 24 h and 48 h, respectively. Conclusion: The results might contribute to the anticancer effect of the curcumin-cisplatin combination in cervical and hepatocellular carcinoma, but in order to support this result and determine its interactions with antineoplastic drugs, further studies are needed.
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