Interaction of Catechins with Human Erythrocytes.

Katarzyna Naparlo,Ireneusz Stefaniuk,Izabela Sadowska-Bartosz,Bogumil Cieniek,Grzegorz Bartosz,Miroslaw Soszynski

doi:10.3390/molecules25061456

Katarzyna Naparlo, Ireneusz Stefaniuk + Show 4 more

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https://doi.org/10.3390/molecules25061456

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Abstract

The aim of this study was to characterize the interaction of chosen catechins ((+)-catechin, (−)-epigallocatechin (EGC), and (−)-epigallocatechin gallate (EGCG)) with human erythrocytes and their protective effects against oxidative damage of erythrocytes. Uptake of the catechins by erythrocytes was studied by fluorimetry, their interaction with erythrocyte membrane was probed by changes in erythrocyte osmotic fragility and in membrane fluidity evaluated with spin labels, while protection against oxidative damage was assessed by protection against hemolysis induced by permanganate and protection of erythrocyte membranes against lipid peroxidation and protein thiol group oxidation. Catechin uptake was similar for all the compounds studied. Accumulation of catechins in the erythrocyte membrane was demonstrated by the catechin-induced increase in osmotic resistance and rigidification of the erythrocyte membrane detected by spin labels 5-doxyl stearic acid and 16-doxyl stearic acid. (−)-Epigallocatechin and EGCG inhibited erythrocyte acetylcholinesterase (mixed-type inhibition). Catechins protected erythrocytes against permanganate-induced hemolysis, oxidation of erythrocyte protein thiol groups, as well as membrane lipid peroxidation. These results contribute to the knowledge of the beneficial effects of catechins present in plant-derived food and beverages.

Highlights

Catechins and their gallate derivatives are a class of flavonoids mainly present in fruits and vegetables and derived products like fruit juices or jams [1]
We studied the protective effect of (+)-catechin and (−)-epigallocatechin gallate on the yeast Saccharomyces cerevisiae with respect to cellular mortality dependent on oxidative stress [17]
We demonstrated that selected monomeric catechins, including (+)-catechin and epigallocatechin gallate (EGCG), protected erythrocytes against oxidative hemolysis induced by 2,20 -azobis(2-amidinopropane) dihydrochloride (AAPH)

Summary

Introduction

Catechins (monomeric flavanols) and their gallate derivatives are a class of flavonoids mainly present in fruits and vegetables and derived products like fruit juices or jams [1]. Monomeric flavanols are abundant in teas derived from the tea plant Camellia sinensis, as well as in some cocoas and chocolates (made from the seeds of Theobroma cacao) [2]. Catechins are present in wine and are found in many other plant species. The main catechins present in green tea, as well as in black tea, are (−)-epigallocatechin (EGC) and the ester of epigallocatechin and gallic acid, (−)-epigallocatechin gallate (EGCG) [4,5]. The food sources of catechins (tea, chocolate, apples, pears, grapes, and red wine) are very popular and highly consumed. Tea catechins consumed by volunteers at a total dose of 240 mg have been found to reach a concentration of up to 0.5 μM in blood plasma [7]

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