Interaction of Beclin 1 with survivin regulates sensitivity of human glioma cells to TRAIL-induced apoptosis

Abstract

We reported a novel interaction between Beclin 1, a key regulator of autophagy, and survivin, a member of the inhibitor of apoptosis protein family. We found that knock-down of Beclin 1 down-regulated survivin protein, and the turnover rate of survivin was increased when Beclin 1 expression was silenced. Knock-down of Beclin 1 sensitized glioma cells to TRAIL-induced apoptosis, and introduction of survivin antagonized the sensitizing effect, suggesting that down-regulation of survivin mediates the enhanced sensitivity to TRAIL-induced apoptosis. These results demonstrate a novel interaction between Beclin 1 and survivin, and may provide a potential mechanism underlying the cross-talk between autophagy and apoptosis. Structured summary MINT- 7969366: Beclin-1 (uniprotkb: Q14457) physically interacts (MI: 0915) with survivin (uniprotkb: O15392) by anti tag coimmunoprecipitation (MI: 0007) MINT- 7968986, MINT- 7969161: survivin (uniprotkb: O15392) physically interacts (MI: 0915) with Beclin-1 (uniprotkb: Q14457) by anti bait coimmunoprecipitation (MI: 0006)