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Abstract
Abstract Fifty greenhouse grown poinsettia plants were infested with all stages of SLW. A total of 7 treatment combinations of BotaniGard (B. bassiana) with various fungicides were compared using 6 replicates (plants) of each treatment. Fungicides were applied twice, 2 d before each of two BotaniGard treatments. Liquids were applied with a hand sprayer, and sulfur (Yellowstone® 99.9% S) was applied by vaporizing (Nivole® sulfur pot, Systems USA, Watsonville, CA) for 6 hours the nights preceding the other fungicide applications. Plants were treated with sulfur in a 113-ft2 greenhouse for either three nights or one night before the first application of BotaniGard; the second sulfur application for both sulfur treatments was only for one night. BotaniGard was applied twice with a 7 d interval, 2 d after fungicide applications. Plants were treated with BotaniGard ES using a 2-gal hand pump sprayer, approximately 25 psi. After treatments, plants were maintained outdoors under a light shade cloth. Three days after treatment with BotaniGard, 2 leaves per plant were collected and pressed on Benomylyeast agar plates and incubated for 24 hours. One hundred spores from each leaf press (12 counts per treatment) were rated as either germinated or not germinated and the percentage of spore germination was calculated. Six days after the last BotaniGard treatment, 2 leaves were collected from each plant and the number of live and dead immature whiteflies in a 4-cm-diam circle were counted on each leaf. Data were analyzed using ANOVA followed by LSD separation of means. Percentage spore germination data and percentage mortality data were arcsin square root transformed before analysis. Number alive counts were square root transformed before analysis. Means are reported as untransformed data.
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