Interaction between the dietary indices and PPAR-γ Pro12Ala gene variants on cardiovascular risk factors in patients with type 2 diabetes mellitus.

Abstract

We investigated the interaction between peroxisome proliferator-activated receptor gamma (PPAR-γ) Pro12Ala polymorphism and healthy eating index (HEI), Dietary Quality Index-International (DQI-I), and dietary phytochemical index (DPI) on cardiovascular disease (CVD) risk factors in patients with type 2 diabetes mellitus (T2DM). This cross-sectional study was conducted on 393 diabetic patients. PPAR-γ Pro12Ala was genotyped by the PCR-RFLP method. Biochemical markers including total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglyceride (TG), superoxide dismutase (SOD), C-reactive protein (CRP), total antioxidant capacity (TAC), pentraxin-3 (PTX3), isoprostaneF2α (PGF2α). Interleukin 18 (IL18), leptin, and ghrelin were measured by standard protocol. Food-frequency questionnaires (FFQ) were used for dietary indices (DQI-I, DPI, HEI) calculation. Homozygous carriers of the rs1801282 C allele showed higher leptin compared G allele carriers (P=.015). The rs1801282-DQI-I interactions were significant on waist circumference (WC) (P=.019). Thus, C-allele carriers in the higher tertile of DQI-I had higher WC compared with GG homozygous. Further, an interaction was observed between PPAR rs1801282 polymorphism and DQI-I on serum IL-18 level (P=.032). Besides, a significant rs1801282-DPI interaction was shown on HDL concentration (P=.041), G allele carriers who were in the highest tertile of DPI, had lower HDL. Moreover, there were significant rs1801282-HEI interactions on serum leptin (P=.021). Individuals with (CC, CG) genotypes in the higher tertile of HEI, had lower leptin concentration. Higher dietary indices (DQI-I, DPI, HEI) may affect the relationship between PPAR-γ Pro12Ala polymorphism and WC, ghrelin, leptin, HDL, and IL-18 concentration in patients with T2DM.