Interaction between saline stress and photoinhibition of photosynthesis in the freshwater green algae Chlamydomonas reinhardtii. Implications for glycerol photoproduction

Abstract

Light intensity is the main limiting factor for the photosynthetic bioconversion of CO2 into glycerol which takes place when Chlamydomonas reinhardtii cells are exposed to saline stress conditions. Although productivity increases with light intensity for low irradiances, a strong inhibition is observed for high light intensity values. Saline stress enhances the damage caused by excess of light on the photosynthetic apparatus. The aim of this work is to evaluate the effect of high light intensity and saline stress on photosynthetic activity, cell growth and glycerol photoproduction by C. reinhardtii. The effect of light intensity on C. reinhardtii cells was studied immediately after transfer to a saline medium and after 24 h of adaptation to saline stress conditions. The influence of light intensity on the glycerol production rate was also evaluated for C. reinhardtii cultured in bioreactors of different radius. The factors that significantly affected photoinhibition were light intensity, cell density, radius of the bioreactor and time of exposure to the high light intensity. Our results suggest that bioreactors with a high surface/volume ratio will enable the achievement of high productivities with relatively low light intensities on the surface and will miminise the photoinhibition effect.