Abstract
Abstract Bacteria and wild yeasts contamination is usually observed during fuel ethanol fermentation. Moreover, it is known that sucrose (the main carbohydrate in sugarcane juice) is breakdown into glucose and fructose by the yeast enzyme invertase, but hydrolysis is much faster than hexoses metabolism, resulting in monosaccharides accumulation which exerts osmotic stress on yeast and growth of Lactobacillus contaminants. Nonetheless, the knowledge about the effect of invertase enzyme on these contaminants is still scarce, especially because the wort composition can differ from exclusive sugarcane juice to a mixture of juice and molasses. Invertase activity and viability of five strains of Saccharomyces cerevisiae (PE-2, BG-1, CAT-1, FLE and IZ-1904) were quantified and correlated with bacterial contamination in fermentations with biomass recycling. Invertase activity was verified to be, in ascending order: CAT-1
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