Abstract
The transient receptor potential vanilloid 1 (TRPV1) receptor is a ligand‐gated cation channel expressed primarilyin sensory nerves and functions as a molecular integrator of sensory input. TRPV1 expressed by sensory neurons also detects salt‐induced changes in tissues and regulates salt sensitivity in hypertension. Sympathetic nerves are closely aligned with sensory nerves in the renal vasculature and sympathetic nerves release ATP that can act on P2Y receptors expressed by sensory nerve fibers. We studied the interaction between P2Y receptors and TRPV1 in kidney specific sensory neurons which are critical for salt balance. Application of Fast Blue (FB) to the nerves surrounding renal artery retrogradely labeled neurons in dorsal root ganglia (DRG, T12‐L4) of rats. Ganglia were harvested and placed in tissue culture for whole cell patch clamp recording from FB‐labeled, kidney‐specific sensory neurons. ATP pretreatment reversed capsaicin (CAP)‐induced TRVP1 desensitization. UTP, a P2Y2 and P2Y4 receptor agonist, also reversed CAP‐induced TRPV1 desensitization. Immunocytochemical studies showed that P2Y1 and P2Y2 receptors were co‐expressed with TRPV1 on FB‐labeled neurons. These data indicate that P2Y2/P2Y4 receptors may function to modulate TRPV1 activity. This may be a mechanism of interaction between sympathetic and sensory nerves supplying the renal vasculature.
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