Abstract

Both nitric oxide (NO) and cold storage have positive effects on the maintenance of fruit quality during storage. However, the roles of NO and storage temperatures in regulating the responses of sphingolipids metabolism to chilling injury of peach fruit during storage remain unknown. Peaches were treated by immersion in distilled water and 15 μmol L−1 NO solution, then stored at 25 °C and 0 °C, respectively. The effects of NO-treatment and storage temperature on the activities of enzymes in sphingolipid metabolism and the contents of sphingolipids in peach fruits were studied. NO maintained higher activities of acid phosphatase (AP) and alkaline phosphatase (ALP) in peach fruits at 25 °C, but promoted the decrease in the activities of AP and ALP at 0 °C, suggesting the regulation by NO on AP and ALP could be modulated by temperature. Compared with the storage at 25 °C, cold storage at 0 °C decreased the activities of phospholipase A (PLA), alkaline phosphatase (ALP), 3-ketodihydrosphingosine reductase (KDSR), sphingosine kinase (SPHK), ceramide synthase (CERS), ceramide kinase (CERK), and the contents of sphingosine (SPH), ceramide (CER), sphingosine-1-phosphate (S1P), ceramide-1-phosphate (C1P), sphingomyelin (SM), and increased the activities of phospholipase C (PLC), phospholipase D (PLD), sphingomyelin synthase (SMS). NO significantly increased the contents of sphingolipid metabolites, and the activities of PLA, KDSR, SPHK, CERS, CERK, but decreased the activities of PLC, PLD, SMS of peaches. The results suggested that NO could maintain sphingolipid metabolism to relieve the response of the postharvest fruit to low temperature.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.