Abstract
Macrophages are cells of the innate immune system that populate every organ. They are required not only for defense against invading pathogens and tissue repair but also for maintenance of tissue homeostasis and iron homeostasis. The aim of this study is to understand whether heme oxygenase (HO) and nitric oxide synthase (NOS) contribute to the regulation of nicotinamide adenine dinucleotide phosphate oxidase (NOX) activity and phagocytosis, two key components of macrophage function. This study was carried out using resting J774A.1 macrophages treated with hemin or vehicle. Activity of NOS, HO, or NOX was inhibited using specific inhibitors. Reactive oxygen species (ROS) formation was determined by Amplex® red assay, and phagocytosis was measured using fluorescein isothiocyanate-labeled bacteria. In addition, we analyzed the fate of the intracellular heme by using electron spin resonance. We show that both enzymes NOS and HO are essential for phagocytic activity of macrophages. NOS does not directly affect phagocytosis, but stimulates NOX activity via nitric oxide-triggered ROS production of mitochondria. Treatment of macrophages with hemin results in intracellular accumulation of ferrous heme and an inhibition of phagocytosis. In contrast to NOS, HO products, including carbon monoxide, neither clearly affect NOX activity nor clearly affect phagocytosis, but phagocytosis is accelerated by HO-mediated degradation of heme. Both enzymes contribute to the bactericidal activity of macrophages independently, by controlling different pathways.
Highlights
Macrophages are cells of the innate immune system that can be found in all tissues
On treatment with the mitochondria-targeted antioxidant mitoTEMPO, total reactive oxygen species (ROS) formation was reduced by 30% (Figure 2A)
We studied the role of heme oxygenase (HO) and nitric oxide synthase (NOS) on ROS generation and phagocytosis, two major tasks of macrophages
Summary
Macrophages are cells of the innate immune system that can be found in all tissues. They are required for maintenance of tissue homeostasis, defense against invading pathogens, tissue repair, and red blood cell recycling. Degradation of the material taken up by phagocytosis is achieved via proteolytic enzymes and facilitated by the so-called oxidative burst. This involves the formation of reactive oxygen species (ROS) and nitric oxide (NO). Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) residing in the phagosomal membrane reduces oxygen (O2) to superoxide anion (O⦁2−) This leads to the formation of hydrogen peroxide (H2O2) and the subsequent generation of highly reactive hydroxyl radicals via the Fenton reaction or the synthesis of hypochlorite by myeloperoxidase. Macrophages are cells of the innate immune system that populate every organ They are required for defense against invading pathogens and tissue repair and for maintenance of tissue homeostasis and iron homeostasis
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