Interaction between culture filtrates of Fusarium culmorum isolates and some root lesion nematodes

Abstract

This study was conducted with root lesion nematodes Pratylenchus penetrans, P. thornei and P. neglectus and three isolates of Fusarium culmorum culture filtrates (ISP, B4, FC5) in vitro conditions. The culture filtrates were diluted 1/0,1/10, 1/20, 1/30, 1/40, 1/50, 1/60, 1/70, 1/80, 90, 1/100 in 1.5 ml microtubes, 250 µl of root lesion nematodes adult+larvae were placed in each microtubes that containing different concentrations of culture filtrate with 50 μl of purified water together with micropipette and incubated at 25±1°C. Nematode mortality were determined after 24 h, 48 h and 72 h. As a result, The mortality effect of culture filtrate of FC5 isolates was determined to be high on three lesion nematode species. Fusarium culmorum culture filtrates of B4 and ISP isolates caused more deaths on P. penetrans than P.thornei and P. neglectus. It has been found to mortality rate increase over time in vitro. The lowest mortality rates were generally found at concentrations of culture filtrates of 1/100 and 1/90, while the highest mortality rates were found at concentrations of 1/0 and 1/10. No difference on P. thornei mortality was detected between the three isolates of F. culmorum culture filtrate at each diluted concentration. In the study, the antagonistic relationship between culture filtrates from F. culmorum isolates and root lesion nematodes were determined in vitro conditions.