Abstract
Mg-ATP was shown to increase the light diffusion of a suspension of rat brain synaptic vesicles (SV) in the presence of rat brain actin-like protein (ALP) (superprecipitation reaction). ALP increases the Mg-ATPase activity of SV and also the liberation of endogenous noradrenalin from SV of bovine hypothalamus, which is abolished by cytochalasin B. Glycolipids (gangliosides and cerebrosides) inhibit the superprecipitation reaction. The results are examined from the standpoint of the contractile hypothesis of mediator secretion.
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