Inter-simple sequence repeat data reveals high genetic diversity in wild populations of the narrowly distributed endemic Lilium regale in the Minjiang River Valley of China.

Zhu-Hua Wu,Ming-Wen Deng,Fu-Xing Jiang,Selvadurai Dayanandan,Jisen Shi,Meng-Li Xi,Randall P Niedz

doi:10.1371/journal.pone.0118831

Zhu-Hua Wu, Ming-Wen Deng + Show 5 more

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https://doi.org/10.1371/journal.pone.0118831

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Journal: PloS one	Publication Date: Mar 23, 2015
Citations: 8	License type: CC BY 4.0

Affiliation: Concordia University, Nanjing Forestry University

Abstract

Lilium regale E.H. Wilson is endemic to a narrow geographic area in the Minjiang River valley in southwestern China, and is considered an important germplasm for breeding commercially valuable lily varieties, due to its vigorous growth, resistance to diseases and tolerance for low moisture. We analyzed the genetic diversity of eight populations of L. regale sampled across the entire natural distribution range of the species using Inter-Simple Sequence Repeat markers. The genetic diversity (expected heterozygosity= 0.3356) was higher than those reported for other narrowly distributed endemic plants. The levels of inbreeding (F st = 0.1897) were low, and most of the genetic variability was found to be within (80.91%) than amongpopulations (19.09%). An indirect estimate of historical levels of gene flow (N m =1.0678) indicated high levels of gene flow among populations. The eight analyzed populations clustered into three genetically distinct groups. Based on these results, we recommend conservation of large populations representing these three genetically distinct groups.

Highlights

Lilies (Lilium spp.) are perennial herbaceous monocots of the family Liliaceae and are commercially important as ornamental plants throughout the world
There was a positive correlation between the MI and polymorphic information content (PIC) values (r2 = 0.909, P
The genetic diversity measures that we found for L. regale were higher than those reported for narrowly endemic plant taxa [20,70,71]

Summary

Introduction

Lilies (Lilium spp.) are perennial herbaceous monocots of the family Liliaceae and are commercially important as ornamental plants throughout the world. Lily bulbs are regularly consumed as food in Asia [1], and some species, including L. longiflorum Thunb., L.browniiF. L. pumilum DC, have been used traditionally in China as sedative, anti-inflammatory and antitussive agents(cough suppressants)[2,3]. The crude extract “Baihe” is prepared from the bulbs of Lilium spp. and is regularly used to treat lung ailments in China [4].

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