Inter-population variation in thermal sensitivity of the tropical toad Duttaphrynus melanostictus, across a small spatial scale in Sri Lanka.

Abstract

Inter-population disparities in a species have been shown to occur as an adaptation to different thermal regimes in the environment. We investigated the thermal sensitivities of the tropical toad Duttaphrynus melanostictus (Asia Common Toad) from two populations at different altitudes: Nuwara-Eliya - 1870m, and Polonnaruwa - 25m, above mean sea level. The two locations were separated by what may be considered a short direct distance - 110km. Thermal sensitivity trials were conducted at six temperatures between 12 and 39°C. Assessments were made using the performance indicators jump distance, jump force, contact time on the test plate following stimulus to jump, and righting time after being overturned. Optimum performance is taken to be the greatest jump distance and jump force, the least contact time on the test plate, and the least righting time. The populations at the two altitudes had markedly different thermal sensitivities - toads in the cool area (Nuwara-Eliya) performed at an optimal level under low temperatures, whereas the toads in the warm area (Polonnaruwa) performed optimally under high temperatures. The finding that the thermal optima (i.e., the temperatures at which the optimal performance for the four performance indicators was recorded) of the toads in Polonnaruwa were below the mean maximum ambient temperature at this location suggests that these toads would be more susceptible to global warming than those in Nuwara-Eliya whose thermal optima were above the mean maximum ambient temperature in that location. This was consistent with the narrower thermal safety margin (i.e., difference between the mean optimum temperature and mean ambient temperature) of toads in Polonnaruwa, compared to those in Nuwara-Eliya. Importantly, these findings demonstrate that, although thermal sensitivity is considered a conservative trait, differentiation does occur even over a small spatial scale presumably because it offers an adaptive advantage to the population concerned.