Abstract

AbstractLaboratory bioassays were done to assess the susceptibility of larvae of the mushroom sciarid fly Lycoriella solani Winnertz (Diptera; Sciaridae) to 16 isolates comprising five species of the entomopathogenic nematode, Steinernema (Nematoda; Steinernematidae). Each isolate was tested by challenging sciarid larvae with single nematodes. Six isolates were also tested in dose‐response experiments. Variations in nematode infectivity occurred (p < 0.001) at both inter‐ and intraspecific levels. Steinernema feltiae (Filipjev) was the most virulent species: two isolates, Nemasys (LD50 = 1.5–3.9) and Sus 94 (1.8–3.9) were superior to the rest. Steinernema kraussei (Steiner) was the least infectious nematode tested as its infection probability never exceeded 0.10. In addition, lethal dose50 values for this species ranged from 9.0–62.6 and two isolates failed consistently to infect any hosts.

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