Inter‐ and intraspecific distribution of an antigenic epitope found in an inbred strain of the Medaka, Oryzias latipes

Abstract

Listen

Translate article

AbstractWe attempted to produce monoclonal antibodies against strain‐specific antigens of the Medaka, Oryzias latipes, using eight inbred strains and several wild populations. One monoclonal antibody, named m11C1, showed reactivity to the cells of one inbred strain HB11C but not to the cells of the other inbred strain HNI. The reactivity was also found for the cells of four other inbred strains but not to that for two inbred strains. To understand the character of the antigen recognized by the m11C1, wer examined wild populations of the Medaka in regard to reactivity for the m11C1. Among fish collected from 122 localities, the m11C1 exclusively recognized the cells of the fish which were classified as the Southern Population. None of the fish from the Northern, the East Korean, and the China‐West Korean Populations reacted with the m11C1 antibody. Thus, the antigen recognized by the m11C1 was named SA1 (Southern Population‐specific Antigen 1). The SA1 antigen from liver cells appeared as a single band on Western blot with a molecular mass of 31 kDa. This antigen mainly localized in epithelia of intestinal vili and in melano‐macrophages which were frequently observed in spleen and anterior kidney. Genetic corsses suggested that SA1‐positive phenotype was dominant. Moreover, SA1 was also detected in two closely related species, O. luzonensis and O. curvinotus, but not in less‐ related five species of the genus Oryzias. Origin and propagation of the SA1 antigen were discussed based on geographic and phylogenic distribution pattern of SA1. © 1993 Wiley‐Liss, Inc.