Abstract
Somatic cell nuclear transfer associated with transgenesis allows the production of animals with beneficial properties that cannot be obtained by conventional breeding programs. Unfortunately these biotechnologies are characterized by a very low efficiency being extremely important to optimize the results of each step from skin biopsy and cloning, until the birth of calf. Animals obtained by cloning have special requirements after birth that must be considered to ensure their survival capacity. Taken into account that there is not much information regarding neonatology of cloned animals, new cases represent a great challenge, and each clinical finding and treatment report should be considered of great value. In this work, we describe all facilities and medical procedures used to ensure the survival of the first bitransgenic bovine clone for human lysozyme and lactoferrin obtained by Somatic Cell Nuclear Transfer (SCNT), born under clinical patterns of Large Offspring Syndrome (LOS). We summarize all maneuvers performed from cesarean section and primary neonatal evaluation, to intensive cares such as sepsis management, internal medium evaluation and correction, and total parental nutrition over a total period of 80 days.
Highlights
Animals produced by cloning commonly have present high birth weight, placental abnormalities and low survival rates, among other conditions, which are grouped under the name of Large Offspring Syndrome (LOS) [1,2]
Due to the heterogeneity of the phenotypes of the animals defined under this syndrome, the name of Abnormal Offspring Syndrome (AOS) has been proposed to reflect the scope of anomalous developmental conditions seen after transference of embryos produced by cloning [3]
We summarize all medical procedures and intensive care used to ensure the survival of the first bitransgenic bovine clone for human lysozyme and lactoferrin production by Somatic Cell Nuclear Transfer (SCNT), born under clinical patterns of LOS/AOS
Summary
Animals produced by cloning commonly have present high birth weight, placental abnormalities and low survival rates, among other conditions, which are grouped under the name of Large Offspring Syndrome (LOS) [1,2]. Due to the heterogeneity of the phenotypes of the animals defined under this syndrome, the name of Abnormal Offspring Syndrome (AOS) has been proposed to reflect the scope of anomalous developmental conditions seen after transference of embryos produced by cloning [3]. Somatic cell nuclear transfer associated with transgenesis allows the obtention of animals that produce valuable proteins for medical and/or nutritional purposes. General information regarding the management of these animals is available, each unique new case represents a great challenge and each every clinical finding and treatment of great value
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