Abstract
Aim.To study morphofunctional properties of platelet lamella′s forming in donors and patients with thrombotic disorders.Materials and methods.We studied platelets of blood donors, patients with deep venous thrombosis, burned patients with thrombotic disorders. Morphofunctional analysis was performed, using original method, based on cell vital staining.Results. In patients with deep venous thrombosis morphofunctional platelet value was enhanced, in burned patients with thrombotic disorders platelet integrity was low. After 10–15 minutes of adhesion on glass spreading platelets maintained 3 subpopulations: cells without lamella, able to fluent granule efflux (1th type); cell with lamellipodias (2th type); cells with wide lamella, covering cell perimeter (3th type). In donors′ blood most of spreading platelets formed lamella at 1–2 hours, in patients with deep venous thrombosis this process was noticeably accelerated, estimating 30–35 minutes, what is more, spreading platelets viewed both intensive lamella′s growth and rapid degranulation of cells without lamella, followed by aggregation on the glass. Burned patients had significant decay of platelet adhesion, lamella formation was low-identified – less than 20 % of spread platelets formed lamellipodias. Among blood donors one could notice heterogeneous level of platelets, capable to rapid granules′ release during contact with adhesive substrate.Conclusion.The lack of lamella forming may occur both at low and high morphofunctional platelet rate. During deep venous thrombosis lamella′s growth velocity was noticeably increased. Among spreading platelets with granules one could find subpopulation of cells, capable to rapid granules′ release in norm and pathology.
Highlights
To study morphofunctional properties of platelet lamella′s forming in donors and patients with thrombotic disorders
We studied platelets of blood donors, patients with deep venous thrombosis, burned patients with thrombotic disorders
У пациентов с тромбозами вен нижних конечностей при адгезии тромбоцитов наблюдается одновременно интенсивный рост ламеллы и быстрая дегрануляция клеток без ламеллы с образованием агрегатов на стекле
Summary
В крови доноров большинство адгезирующих тромбоцитов первого и второго типов формировали обширную ламеллу через 1–2 часа адгезии, у пациентов с тромбозами вен – через 30–35 минут. Среди доноров крови выявлена неоднородность по уровню тромбоцитов, способных к быстрому выбросу гранул при контакте с адгезивным субстратом. We studied platelets of blood donors, patients with deep venous thrombosis, burned patients with thrombotic disorders.
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