Abstract
BackgroundCondyloma acuminatum (CA) is one of the most common sexually transmitted diseases and induced by low-risk human papillomaviruses (HPVs), mainly HPV type 6 and 11. Here, we report the identification of (-)-Epigallocatechin-3-gallate (EGCG) by an HPV11 infection cell model.ResultsThe recombined HPV11.HaCaT cells had stable HPV 11 early genes expression. The introducing of HPV11 genome significantly increased the proliferation of HPV11.HaCaT cells, as well as the proportion of cells in S and G2/M phases. After treated with rhIFN-α 2a, IFN signaling pathway was activated in both HaCaT and HPV11.HaCaT cells, while HPV11 decreased the activation level. In addition, rhIFN-α 2a, could inhibit expression of HPV 11 E6 and E7 mRNA significantly (P<0.05). However, cell growth and cell cycle did not show statistical difference (P>0.05). Nevertheless, EGCG, a major active constituent in tea polyphenol, showed strong anti-HPV11 effect, which inhibited HPV11 E6 and E7 mRNA.MethodsGene transfection technique was used to introduce HPV11 genome into HaCaT cells, named HPV11.HaCaT cells. With the established cell model, we explore the anti-HPV11 effect of (-)-Epigallocatechin-3-gallate (EGCG) on cell growth, viability and affection on expression HPV11 E6 and E7 mRNA.ConclusionOur data collectively demonstrated that the recombinant HPV11.HaCaT cells were integral and practical to be a cell model to test anti-HPV11 agents and explore the interaction between HPV11 genes and host cells. And EGCG inhibits expression of HPV11 E6 and E7 mRNA in the recombinant HPV11.HaCaT cells.
Highlights
human papillomaviruses (HPVs) cause a number of diseases ranging from benign lesions to malignant tumors
Our data collectively demonstrated that the recombinant HPV11
To explore the translation of HPV early genes into proteins, related proteins were detected by commercialized antibodies against HPV11early genes, i.e. anti-HPV11 E7 antibody
Summary
HPVs cause a number of diseases ranging from benign lesions to malignant tumors. According to different pathogenicity, HPVs can be divided into “highrisk” groups (HR HPVs) and “low-risk” groups (LR HPVs). Comparing to HR HPVs, LR HPVs mainly induce benign lesions, such as verruca vulgaris, flat warts and condyloma acuminatum (CA). LR HPVs are correlated with clinical outcome of oral cavity squamous cell carcinoma [2]. CA, which mainly caused by HPV type 6 and 11, is a common sexually transmitted disease and the incidence is rising year by year all over the world. Drugs used locally for CA, including imiquimod, interferon, podophyllotoxin, etc., are focused on immunomodulating or cytotoxicity, not against HPVs. In addition to increasing incidence, the high recurrence rate of CA has makes the disease become one of the common sexual transmitted disease affecting the public health problems. Condyloma acuminatum (CA) is one of the most common sexually transmitted diseases and induced by low-risk human papillomaviruses (HPVs), mainly HPV type 6 and 11. We report the identification of (-)-Epigallocatechin-3-gallate (EGCG) by an HPV11 infection cell model
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