Abstract
The aim of this study was to elucidate the role of the integrin-linked kinase (ILK) gene in development of human bladder transitional cell carcinoma (BTCC). Expression of ILK protein and ILK mRNA in 56 cases of human BTCC tissue and in 30 cases of adjacent normal bladder tissue was detected by immunohistochemistry S-P and reverse transcription polymerase chain reaction (RT-PCR), respectively. Four specific miRNA RNAi vectors targeting human ILK were synthesized and transfected into BIU-87 cells by liposome to obtain stable expression cell strains. The influence of ILK on proliferation of BTCC was detected by MTT, FCM on athymic mouse tumorigenesis. The positive rate of ILK protein in BTCC tissue (53.6%) was much higher than adjacent normal bladder tissue (10.0%) (p<0.05). Similarly, expression of ILK mRNA in BTCC tissue (0.540 ± 0.083) was significantly higher than in adjacent normal bladder tissue (0.492 ± 0.070) (p<0.05). MTT showed that the proliferation ability of miRNA-ILK transfected group was clearly decreased (p<0.05), the cell cycle being arrested in G0/G1-S, an tumorigenesis in vivo was also significantly reduced (p<0.05). ILK gene transcription and protein expression may be involved in the development of BTCC, so that ILK might be the new marker for early diagnosis and the new target for gene treatment.
Highlights
Bladder carcinoma is one of the common malignant tumors
Expression of Integrin-linked kinase (ILK) protein and ILK mRNA in 56 cases of human bladder transitional cell carcinoma (BTCC) tissue and in 30 cases of adjacent normal bladder tissue was detected by immunohistochemistry S-P and reverse transcription polymerase chain reaction (RT-PCR), respectively
Expression of ILK mRNA in BTCC tissue (0.540±0.083) was significantly higher than in adjacent normal bladder tissue (0.492±0.070) (p
Summary
Bladder carcinoma is one of the common malignant tumors. An estimated 386, 300 new cases and 150, 200 deaths from bladder cancer occurred in 2008 world wide. Bladder carcinoma is a complex multi-step process involving the activation and/ or abrogation of signal transduction pathways resulting in a variety of cellular activities. A series of studies had found that ILK abnormally over-expressed and was activated in prostate cancer (Kieffer et al, 2005), ovarian cancer (Ahmed et al, 2003), colon cancer (Marotta et al, 2003), gastric cancer (Ito et al, 2003) and other multi-malignant tumors, ulitizing many mechanism: such as inhibiting cell apoptosis, and promoting cell proliferation, these changes move forward a single step to affect biological function (Gu et al, 2012), integrin and other element enhance tumor tissue invasion force through the endothelial cells of the adhesion strength changes (Kaiser et al, 2012). The expression of ILK in the BTCC and its role in occurrence and development of BTCC are still unclear
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