Integrin expression during long-term cultivation in multipotent mesenchymal stromal cells isolated from human adipose tissue

Abstract

Integrin expression during long-term cultivation in multipotent mesenchymal stromal cells (MMSCs) isolated from human subcutaneous adipose tissue was studied. It was found that expression of α1, α4 and α6 integrins at the 2nd and 17th passages was different. The cell number positively stained with antibodies against CD49a (α1 integrin) and CD49d (α4 integrin) dropped to 87.2 and 11.2%, respectively. On the contrary, the cell number stained with antibodies against CD49f (α6 integrin) and CD49b (α2 integrin) increased during long-term cultivation by 9.9 and 2.3%, respectively. The high expression of β1 integrin (98%) did not significantly change during MMSC cultivation. Induction of MMSC to osteogenic differentiation (formation of bone tissue cells and extracellular matrix) was more efficient at the 2nd than the 17th passage. Thus, long-term MMSC cultivation required for cell-mass accumulation affects the cell functions. It should be considered for their application in cellular technology, including tissue modeling in the three-dimension scaffold.