Abstract
The genotypes of intracellular phage DNA molecules have been determined by transfection of the DNA and genetic analysis of the resulting mature phage. This method has been used to investigate several factors affecting the formation of recombinant DNA by the site-specific λ integration system. It has been shown that: (1) all gene products needed for integrative recombination can be supplied in trans; none needs to be made by the recombining DNA molecule. (2) These gene products are functionally stable at 31 °C but are rapidly inactivated at higher temperatures. (3) Interference with genetic expression of the parental DNA by inhibitors or phage repressor alters the kinetics of recombination by causing an early appearance of integrative recombinant DNA. Several possible explanations for this phenomenon are discussed. (4) Integrative recombination is strongly inhibited by cyanide or dinitrophenol. (5) xis gene product has an apparent inhibitory effect on integrative recombination in cells treated with inhibitors of transcription and translation.
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