Abstract
This work was supported by grants from the Spanish Government (BIO2001-1721) and the Generalitat of Catalunya (2014SGR-1434).
Highlights
High-throughput “-omics” technologies have enabled global measurement of biological molecules (DNA, RNA, proteins, metabolites, etc.) and their interactions, producing network modules
In view of the potential fundamental roles of the GID/MRCTLH protein complexes in plants there is a great need for better understand the relationships of the protein components of the complex in plants. To this end we have identified the individual components of the GID/MRCTLH complex encoded by the Arabidopsis genome and we have examined their possible protein-protein interactions to determine the potential structure of the GID/MRCTLH complexes in plants and their potential roles
In order to identify the proteins in Arabidopsis thaliana which are homologues to the proteins in the GID/MRCTLH complex we searched by BLASTP method against the Arabidopsis protein sequences
Summary
High-throughput “-omics” technologies have enabled global measurement of biological molecules (DNA, RNA, proteins, metabolites, etc.) and their interactions, producing network modules. The first step required to perform protein-protein interaction network analysis is to identify the proteins of interest. The second step is to use the identified proteins to search and retrieve binary interactions from a curated proteinprotein interaction databases. A network can be assembled based on the set of interactions. The GID/MRCTLH protein complex was first discovered in yeast (S. cerevisiae), where it was named GID (glucoseinduced degradation deficient)(Regelmann et al, 2003). GID complex is a 600 kDa assembly of seven proteins (GID1 (VID30), GID2, GID4 (VID24), GID5 (VID28), GID7, GID8 and GID9). Many of the proteins in the GID complex contain LisH and CTLH domains
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