Abstract
AbstractThe striatum is a brain area with a high density of dopaminergic terminals and so it is considerably affected when dopaminergic neurotoxicity occurs. Several methods have been developed to evidence such neurotoxicity, some of which can be performed in vitro, using membranes or synaptosomes obtained from fresh tissues. Concretely, here we detail our methodological experience assessing reactive oxygen species in striatal synaptosomes, as well as measuring brain terminal damage after a neurotoxic treatment leading to decreased [3H]WIN 35428 binding and tyrosine hydroxylase expression measured by Western blot. Also, we have assessed impairment of dopamine transporter after dopaminergic neurotoxicity measuring uptake of [3H]dopamine in striatal synaptosomes. These techniques are complementary and can be useful to assess the dopaminergic neurotoxic potential of drugs such as amphetamine derivatives, among others.Key wordsDopamine uptakeDopaminergic neurotoxicityReactive oxygen speciesStriatumSynaptosomesTyrosine hydroxylase[3H]WIN 35428 binding
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
