Integrative Analysis of the Expression of microRNA, Long Noncoding RNA, and mRNA in Osteoarthritis and Construction of a Competing Endogenous Network.

Abstract

This study aimed to identify potential core microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and mRNAs in osteoarthritis (OA) to construct a competing endogenous RNA (ceRNA) and co-expression network. Differentially expressed miRNAs (DEMis) in the dataset GSE143514 comprising five OA and three normal tissues were identified using the DEseq package. Core miRNAs were identified as DEMis overlapping with those reported by the human microRNA disease database. LncRNAs were predicted by the miRNA-lncRNA interactions network from the encyclopedia of RNA interactomes (ENCORI). MiRNet and ENCORI were employed to predict the mRNAs which overlapped with the differentially expressed mRNAs from the dataset GSE114007 to obtain overlapping mRNAs. MiRNA-lncRNA and miRNA-mRNA interactions were integrated to construct the ceRNA network. A total of 143 DEMis were identified in OA and normal tissues, from which hsa-miR-451a, hsa-miR-370-5p, hsa-miR-34a-5p, hsa-miR-210-3p, and hsa-miR-101-3p were assessed as core miRNAs using overlapping analyses. These RNAs may represent potential therapeutic targets for the treatment of OA.