Abstract
Endometrial carcinoma (EC) is the fifth widely occurring malignant neoplasm among women all over the world. However, there is still lacking efficacy indicators for EC's prognosis. Here, we analyzed two databases including an RNA-sequencing-based TCGA dataset and a microarray-based GSE106191. After normalizing the raw data, we identified 114 common genes with upregulation and 308 common genes with downregulation in both the TCGA and GSE106191 databases. Bioinformatics analysis showed that the differently expressed genes in EC were related to the IL17 signaling pathway, PI3K-Akt signaling pathway, and cGMP-PKG signaling pathway. Furthermore, we performed the least absolute shrinkage and selection operator (LASSO) Cox regression analysis and generated a signature featuring 17 prognosis-related genes (MAL2, ANKRD22, METTL7B, IL32, ERFE, OAS1, TRPC1, SRPX, RAPGEF4, PSD3, SIMC1, TRPC6, WFS1, PGR, PAMR1, KCNK6, and FAM189A2) and found that it could predict OS in EC patients. The further analysis showed that OAS1, MAL2, ANKRD22, METTL7B, and IL32 were significantly upregulated in EC samples after comparison with normal samples. However, TRPC1, SRPX, RAPGEF4, PSD3, SIMC1, TRPC6, WFS1, PGR, PAMR1, KCNK6, and FAM189A2 were significantly downregulated in EC samples in comparison with normal samples. And correlation analysis showed that our results showed that the expressions of 17 prognosis-related hub genes were significantly correlated based on Pearson correlation. We here offer a newly genetic biomarker for the prediction of EC patients' prognosis.
Highlights
Endometrial carcinoma (EC) is the fifth commonly occurring malignant neoplasm among women all over the world, with an estimated 382,000 new EC cases and nearly 90,000 deaths in 2018 [1, 2]
We identified 30 Differentially Expressed Genes (DEGs) that were related to the prognosis of EC, including KCNK6, IL32, FAM189A2, WFS1, GREB1, WFDC1, PGR, PAMR1, TRPC6, ADAM28, ANKRD22, GLDC, RAPGEF4, MCM10, TRO, OAS1, BEX4, PSAT1, METTL7B, TIMP3, FBXO17, PTTG1, POLQ, MAL2, SIMC1, ERFE, TRPC1, SRPX, UST, and PSD3
We identified that 30 DEGs were related to the prognosis of EC, comprising KCNK6, IL32, FAM189A2, WFS1, GREB1, WFDC1, PGR, PAMR1, TRPC6, ADAM28, ANKRD22, GLDC, RAPGEF4, MCM10, TRO, OAS1, BEX4, PSAT1, METTL7B, TIMP3, FBXO17, PTTG1, POLQ, MAL2, SIMC1, ERFE, TRPC1, SRPX, UST, and PSD3
Summary
Endometrial carcinoma (EC) is the fifth commonly occurring malignant neoplasm among women all over the world, with an estimated 382,000 new EC cases and nearly 90,000 deaths in 2018 [1, 2]. In the United States, it is speculated that the number of newly diagnosed ECs will be increasing over time. It is estimated that the occurrence rate is still rising with increasing risk factors for certain ECs, including obesity rate and the aging of the US population [3]. The incidence rate of EC increases after the age of 30, and the peak incidence is within 60 to 69 years. 20% to 30% of patients with EC are diagnosed in the advanced stage during surgery. The five-year survival rate of patients in stage III ranged from 40% to 70% and in stage IV was within 0 to 10% [1, 4]. Despite therapeutic advances having been made, high recurrence rate and metastasis remain to be big challenges [4]. To determine effective therapeutic strategies in ameliorating the prognostic status of EC patients is essential
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