Integration of UV Spectrophotometry Method For Simultaneous Estimation of Fluconazole and Efinaconazole in Nanoparticle Loaded Hydrogel

Abstract

The goal of this study was to create and validate a UV spectrophotometric method for fluconazole and efinaconazole. Ultraviolet spectroscopy was employed at 255 nm for fluconazole and 268 nm for efinaconazole, using samples prepared in a DMF and phosphate buffer solution with a pH of 4.9. The method demonstrated strong linearity with a correlation coefficient of 0.999. Following ICH guidelines, the validity of the method was assessed for various parameters, including accuracy, precision, limit of detection (LoD), limit of quantification (LoQ), recovery study, and range. The simplicity, time efficiency, and cost-effectiveness of UV spectroscopy were key factors in its selection over HPLC technique, which is known for being expensive and time-consuming with potential susceptibility to various factors affecting determination. Through repeated experiments and meticulous sampling, the method exhibited linearity, accuracy, repeatability, and lack of errors. It was also found to be selective, specific, and cost-effective, affirming its reliability. Moreover, the use of a consistent solvent throughout the experimental work ensured that the method was free from interference by any excipients. Overall, the proposed UV spectrophotometric method was deemed simple, rapid, precise, accurate, and sensitive, making it suitable for routine analysis of fluconazole in both single and combined forms