Integration of three non-interfering SERS probes combined with ConA-functionalized magnetic nanoparticles for extraction and detection of multiple foodborne pathogens.

Abstract

Asandwich-structured SERS biosensor has been constructedfor simultaneous detection of multiple pathogenic bacteria, consisting of non-interfering SERS probes for bacterial labeling and ConA-functionalizd magnetic nanoparticles for bacteria extraction. A the preparation method of PP3 SERS probe with high Raman activity isreported for the first time. Since the PP3 SERS probe has a very strong Raman peak at 2081 cm-1 in the "Raman silent region," the mixed SERS probe formed with MP1 and DP2 can meet the needs of multiple foodborne pathogen detection. Significantly, S. aureus, E. coli, and P. aeruginosa can be successfully extracted upon external magnetic field, and the limit of detection (LOD) is 1 CFU‧mL-1, lower than that ofthe congeneric detectors. This work paves a new way for the construction of anovel detector and absorbent for different bacteria in complex samples by using SERS probe.