Integration of sustainable chemistry frameworks with a single-pot, on-off fluorescence approach for enabling the facile quantification of mosapride; Application to pharmaceutical tablets and content uniformity

Abstract

The twelve green chemistry concepts were considered for designing two new feasible, sensitive and selective spectroscopic approaches for the estimation of mosapride (MSP) in pure powder and tablet dosages. These approaches were derived from the reaction of the drug with erythrosine B in buffered acidic medium. The reaction yielded a binary complex, and its absorbance can be measured at its λmax (556 nm) while the incorporation of erythrosine B in the complexation reaction caused quantitative quenching of its native fluorescence that could be measured at 554 nm after excitation at 529 nm. The reaction parameters were carefully optimized for the two methods then the calibration curves were plotted. The absorbance and fluorescence quenching were directly proportional to MSP concentration in the ranges of 1.0–12.0 and 0.15–2.0 μg/mL, respectively. Limits of detection and quantitation were estimated giving values of 0.30 and 0.92 μg/mL for spectrophotometric method and 0.047 and 0.143 μg/mL for spectrofluorimetric method. In accordance with the International Council for Harmonization (ICH) guiding rules, the new methods were assessed proving their high accuracy, precision, and robustness. The methods were adopted for the determination of the cited drug in pharmaceutical tablets. Moreover, the uniformity of tablet contents was successfully tested according to USP utilizing the suggested approaches. Furthermore, the greenness of the suggested procedure was confirmed using three different approaches.