Abstract
BackgroundRapeseed (Brassica napus, B. napus) is an important oil seed crop in the world. Previous studies showed that seed germination vigor might be correlated with seed oil content in B. napus, but the regulation mechanism for seed germination has not yet been explained clearly. Dissecting the regulation mechanism of seed germination and germination vigor is necessary.ResultsHere, proteomic and genomic approaches were used to analyze the germination process in B. napus seeds with different oil content. The identification of 165 differentially expressed proteins (DEPs) in the germinating seeds of B. napus with high and low oil content was accomplished by two-dimensional fluorescence difference in gel electrophoresis (2D-DIGE). The comparative proteomic results revealed that seeds with high oil content had higher metabolic activity, especially for sulfur amino acid metabolism. Thirty-one unique genes were shown to be significantly changed during germination between the seeds with high and low oil content, and thirteen of these genes were located within the confidence interval of germination-related quantitative trait locus (QTLs), which might play an important role in regulating seed germination vigor.ConclusionsThe present results are of importance for the understanding of the regulation mechanism for seed germination vigor in B. napus.
Highlights
Rapeseed (Brassica napus, B. napus) is an important oil seed crop in the world
Effects of oil content on seed germination vigor in B. napus To verify whether the oil content could affect the seed germination vigor, 10 cultivars with high oil content and 10 with low oil content were used for the germination experiment
It was revealed that some seeds began to germinate at 12 h after imbibition (HAI), and most had completed germinating at 32 HAI
Summary
Rapeseed (Brassica napus, B. napus) is an important oil seed crop in the world. Previous studies showed that seed germination vigor might be correlated with seed oil content in B. napus, but the regulation mechanism for seed germination has not yet been explained clearly. Dissecting the regulation mechanism of seed germination and germination vigor is necessary. Research on the regulation mechanism of seed germination is important for agricultural production. The seed germination and germination vigor are affected by many factors in B. napus, such as salt [4, 5], temperature [6, 7], plant hormones [8], and aging [9]. Jian et al identified 19 QTLs that are associated with seed germination rates under salt and drought stress in B. napus [17]
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