Abstract
An optofluidic device is demonstrated with photonic components integrated onto the chip for use in fluorescence and scatter detection and counting applications. The device is fabricated by integrating the optical and fluidic components in a single functional layer. Optical excitation on-chip is accomplished via a waveguide integrated with a system of lenses that reforms the geometry of the beam in the microfluidic channel into a specific shape that is more suitable for reliable detection. Separate counting tests by detecting fluorescence and scattered signals from 2.5 and 6.0 μm beads were performed and found to show detection reliability comparable to that of conventional means of excitation and an improvement over other microchip-based designs.
Highlights
Microchip based devices have the potential to expand the scope of applications for diagnostic and clinical procedures to point-of-care settings and remote and on-line sensing situations
Devices exploit cost savings in two ways: via economies of scale from large volumes of production of simple and inexpensive devices for very specific applications, and from reductions in required samples and associated dyes for fluorescent labeling. These lab-on-a-chip (LOC) devices have been demonstrated in point of care (POC) medical and remote or on-site applications ranging from diagnosing and monitoring HIV infections [1], immunoassays [2], portable hematology
The method for integrating excitation optics and the process of careful beam shaping demonstrated in these devices are suited for applications in POC and remote settings where conventional cytometry methods for excitation are too expensive or cumbersome for efficient operation
Summary
Microchip based devices have the potential to expand the scope of applications for diagnostic and clinical procedures to point-of-care settings and remote and on-line sensing situations. Devices exploit cost savings in two ways: via economies of scale from large volumes of production of simple and inexpensive devices for very specific applications, and from reductions in required samples and associated dyes for fluorescent labeling These lab-on-a-chip (LOC) devices have been demonstrated in point of care (POC) medical and remote or on-site applications ranging from diagnosing and monitoring HIV infections [1], immunoassays [2], portable hematology. The diversity of microchip based devices has been demonstrated in applications such as counting leukocytes [6], detecting bacterial contamination in milk [7], detecting tumor cells [8], the immunological differentiation of leukocytes [9], among many others [10,11] Successful commercialization of these devices will rely on the ability to integrate components on chip— the optical excitation and detection components. Devices will be manufactured to complete a specific analytical application that requires as little time, training, or cost as possible to operate
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