Integration of Magnetic Capture and SERS Signal Probes for Sensitive Competitive Aptamer-based Detection of Cardiac Troponin I

Abstract

Abstract In this study, a high sensitivity bimetallic SERS substrate Au@Ag is introduced with an EF of about 2.03 × 1011 to construct a competitive SERS aptasensor integrated aptamer and magnetic capture for detection of cardiac troponin I (cTnI). The aptasensor shows low quantitative concentration of detection (0.01 ng/mL), great linear relationship (R2 = 0.993) between SERS intensity and Log value of cTnI concentration (0.01 ng/mL–1.00 ng/mL). The proposed method is a rapid and sensitive detection of SERS aptasenor for cardiac troponin I, which has great application potential in clinic detection.