Abstract
We are developing microfabricated devices consisting of arrays of electrochemical electrodes in order to increase the throughput of single-cell measurements of quantal exocytosis from neuroendocrine cells and to develop technology that allows simultaneous electrochemical detection and fluorescence imaging of single fusion events. One component of this effort is to develop on-chip methods for stimulating exocytosis from select cell population on the chip. Here we describe our efforts to use the same electrode to electrically stimulate the adjacent cell and subsequently measure exocytosis using amperometry.
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