Abstract
A fluorescence-detection system for immunoassay applications in a laminate-based microfluidic chip has been developed and tested. Blue-LED, plastic fiber, CCD detector, mylar-D, and fluorescein are used as an excitation source, a light coupler, a fluorescence detector, a microfluidic substrate, and a sample, respectively. The system is able to detect from 1 to 1000 μg/L fluorescein. A method to avoid cross talk is also presented. © 2005 Wiley Periodicals, Inc. Microwave Opt Technol Lett 45: 456–460, 2005; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.20852
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