Integrating Molecular Network and Culture Media Variation to Explore the Production of Bioactive Metabolites by Vibrio diabolicus A1SM3.

Natalia Conde-Martínez,Norberto Lopes,Anelize Bauermeister,Alan Pilon,Edisson Tello

doi:10.3390/md17040196

Natalia Conde-Martínez, Norberto Lopes + Show 3 more

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https://doi.org/10.3390/md17040196

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Abstract

Vibrio diabolicus A1SM3 strain was isolated from a sediment sample from Manaure Solar Saltern in La Guajira and the produced crude extracts have shown antibacterial activity against methicillin-resistant Staphylococcus aureus and cytotoxic activity against human lung cell line. Thus, the aim of this research was to identify the main compound responsible for the biological activity observed and to systematically study how each carbon and nitrogen source in the growth media, and variation of the salinity, affect its production. For the characterization of the bioactive metabolites, 15 fractions obtained from Vibrio diabolicus A1SM3 crude extract were analyzed by HPLC-MS/MS and their activity was established. The bioactive fractions were dereplicated with Antibase and Marinlit databases, which combined with nuclear magnetic resonance (NMR) spectra and fragmentation by MS/MS, led to the identification of 2,2-di(3-indolyl)-3-indolone (isotrisindoline), an indole-derivative antibiotic, previously isolated from marine organisms. The influence of the variations of the culture media in isotrisindoline production was established by molecular network and MZmine showing that the media containing starch and peptone at 7% NaCl was the best culture media to produce it. Also, polyhydroxybutyrates (PHB) identification was established by MS/MS mainly in casamino acids media, contributing to the first report on PHB production by this strain.

Highlights

Vibrionaceae family is considered a Gram-negative and gammaproteobacteria, ubiquitous in marine environments and has been spotted out for its genomic flexibility and mainly for their importance as pathogens [1]
Considering that the antibacterial and cytotoxic activity of Vibrio diabolicus A1SM3 was established in our group [17], we proposed here a strategy combining different growth parameters with molecular network tool for rapid and reliable clustering and characterization of bioactive compounds
Among the growth media tested at different salinities, it was possible to establish that the best growth of Vibrio diabolicus A1SM3 was at 4% NaCl, the same salinity of the pond from it was isolated in Manaure Solar Saltern

Summary

Introduction

Vibrionaceae family (composed c.a. 128 species) is considered a Gram-negative and gammaproteobacteria, ubiquitous in marine environments and has been spotted out for its genomic flexibility and mainly for their importance as pathogens [1]. Until 2011, only 93 bioactive compounds have been reported to be isolated from the Vibrionaceae family [2]. Some studies revealed the potential of Vibrionaceae as a source of new and bioactive natural products [3]. Vibrio spp. are known to be the most representative species in the Vibrionaceae family (64 species) being characterized by the non-ribosomal peptides production and some hybrids synthesized by non-ribosomal peptide and polyketide synthases [2,4]. Another family of bioactive compounds that have been widely reported is.

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