Abstract
In vitro methods provide a variety of tools to supplement traditional methods for collecting, propagating and preserving endangered plant species (Henshaw and Blakesley, 1996; Pence, 1999; Sugii and Lamoureux, 2004). These methods are in use by researchers in several programs around the world, including at the Center for Conservation and Research of Endangered Wildlife (CREW) at the Cincinnati Zoo & Botanical Garden, where they are used to address reproductive challenges in a variety of United States endangered species. In vitro methods also provide a supplemental approach for addressing Target 8 of the Global Strategy for Plant Conservation (Wyse Jackson, 2004), when traditional methods of ex situ conservation are not adequate.
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