Integrating field-based heat tents and cyber-physical system technology to phenotype high night-time temperature impact on winter wheat

Abstract

BackgroundMany agronomic traits have been bred into modern wheat varieties, but wheat (Triticum aestivum L.) continues to be vulnerable to heat stress, with high night-time temperature (HNT) stress shown to have large negative impact on yield and quality. Global mean temperature during the day is consistently warming with the minimum night temperature increasing at a much quicker pace. Currently, there is no system or method that allows crop scientists to impose HNT stress at key developmental stages on wheat or crops in general under field conditions, involving diverse genotypes and maintaining a dynamic temperature differential within the tents compared to the outside.ResultsThrough implementation of a side roll up and a top ventilation system, heaters, and a custom cyber-physical system using a Raspberry Pi, the heat tents were able to consistently maintain an elevated temperature through the night to differentiate heat stress impact on different genotypes. When the tents were placed in their day-time setting they were able to maintain ambient day-time temperature without having to be removed and replaced on the plots. Data averaged from multiple sensors over three consecutive weeks resulted in a consistent but small temperature difference of 0.25 °C within the tents, indicating even distribution of heat. While targeting a temperature differential of 4 °C, the tents were able to maintain an average differential of 3.2 °C consistently throughout the night-time heat stress period, compared to the outside ambient conditions. The impact of HNT stress was confirmed through a statistically significant yield reduction in eleven of the twelve genotypes tested. The average yield under HNT stress was reduced by 20.3% compared to the controls, with the highest reduction being 41.4% and a lowest reduction of 6.9%. Recommendations for fine-tuning the system are provided.ConclusionThis methodology is easily accessible and can be widely utilized due to its flexibility and ease of construction. This system can be modified and improved based on some of the recommendations and has the potential to be used across other crops or plants as it is not reliant on access to any hardwired utilities. The method tested will help the crop community to quantify the impact of HNT stress, identify novel donors that induce tolerance to HNT and help the breeders develop crop varieties that are resilient to changing climate.