Abstract
The simultaneous recording of fluorescence intensities at two different wavelengths has a variety of applications in optical microscopy, particularly for ratiometric measurements to sense environmental conditions or for measuring fluorescence resonance energy transfer to probe protein conformation. We demonstrate that a Czerny–Turner monochromator can serve as a platform for dual-color imaging by inserting into the monochromator turret a glass wedge with different dichroic coatings on each side. The presence of a grating, a mirror, and the wedge on a single turret allows rapid switching between spectroscopy and different imaging modes, and the reflective optics in the monochromator minimizes chromatic aberrations.
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